The Massachusetts Biomanufacturing Center at the University of Massachusetts Lowell (UML) occupies a more than 3000 square feet lab. The lab space is shared by four PIs: Prof. Carl Lawton, Prof. Seongkyu Yoon, Prof. Jin Xu, and Prof. Dongming Xie. All labs in the center are well maintained by technical staff and available for the research and training at any times.
Floor model microbial shaker/incubators (3), tabletop water bath shaker/incubators (2), New Brunswick Scientific Celligen Plus 5 L cell culture reactor (1), New Brunswick BioFlo 100 1.3 L cell culture reactors (2), New Brunswick Scientific SF-116 16 L fermenters (3), Sartorious Biostat B+ – 2 L microbial fermentor with data logging (4), Sartorius A+ 5 L cell culture reactors with data logging (2), 200 L microbial reactor with clean steam, high purity water, and gases (1), Invensys Fusion Automation and Data Acquisition system (1). New Sartorius Biostat B+ Twins (2L and 5L) fermentors will be added soon.
4 ft biosafety cabinets (2), SDS-PAGE/Western Blot (XCell II/ Invitrogen), CO2 incubators with multi-position stir plates (3), Nexcelom Cellometer Auto T4 (total and viable cell counts) (1), CEDEX HiRes Cell counter (1), CEDEX BIO metabolic analyzer (1), Nova Biomedical BP 400 with autosampler (1), Inverted microscope (1), Phase contrast microscope (1). Near Infrared spectroscopy (Bruker Optics) (1), Tube-welder (Turumo) (1), thermocyclers for PCR (2), DNA Gel electrophoresis. More thermocyclers for PCR, DNA and protein Gel electrolysis, will also be added soon.
High-speed Centrifuge (Allegra X-22R/Beckman Coulter), Separagen QuantumSup Chromatography system and data logging (10 ml/min-100ml/min) (1), Millipore K-Prime 40-I chromatography system and data logging (100ml/min – 500 ml/min) (1), 70 mm Quikscale columns (2), 100 mm QuikScale columns (2), Millipore Cogent mScale TFF automated system (2), Millipore MPodPilot filtration system (1), Millipore Pellicon system (1), 6 L refrigerated centrifuge (1), 3 L refrigerated centrifuge (1), Microfluidics M-110EH homogenizer (1).
UV/Vis (Lambda 25/PerkinElmer), Fluorescence Spectrophotometer (LS55/PerkinElmer), HPLCs with UV/Vis/Fluorescence Detector (Alliance/Waters) (4), Fluorescence/ Chemiluminescence Imaging System (AlphaImager HP/AlphaInnotech), Mass Spec (ESI-TOF/Waters), SDS-PAGE/Western Blot (XCell II/Invitrogen), High-speed Centrifuge (Allegra X-22R/Beckman Coulter), Multiangle Laser Light Scattering (MiniDawn/Wyatt Technologies), CD Spectrophotometer (J-810/Jasco).
